Contact an Account ManagerSimultaneous determination of verapamil and its seven metabolites by HPLC
Summary
Type: HPLC
Application #: 104710
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: fluorescence, excitation 203nm, emission 320nm
Conditions: eluent: phosphate buffer pH 4.6, 2g/l Na 1-heptanesulphonate - acetonitrile (1:1, v/v)
flow rate 1.5ml/min
Authors: Kuwada M
Source: J Chromatogr 222 (1981) 507 - 511
Details
Legend: Fig. 1. Chromatograms of extracts of (A) 1ml blank plasma, (B) 1ml plasma
spiked with 25ng internal standard (9) and 31.25ng each of verapamil and 7
metabolites, (C) a mixture of 25ng int. std. and